Issue 1 (199), article 5

DOI:https://doi.org/10.15407/kvt199.01.085

Cybernetics and Computer Engineering, 2020, 1(199)

Azarchov O.Yu.1, DSc. (Medicine),
Head of the Biomedical Engineering Department
e-mail: alexazarhov@gmail.com

Chernyshova T.A.2, Physician,
e-mail: tetyana.che@gmail.com

1Pryazovsky State Technical University of the Ministry of Education and Science of Ukraine,
7, University st., Mariupol, 87555, Ukraine

2Aviation Medical Center of the National Aviation University,
1, Komarova av., Kyiv, 03058, Ukraine

APPLICATION OF INFORMATION TECHNOLOGY FOR DETERMINATION OF CIRCULATING TUMOR CELLS TO DIAGNOSTICS OF MALIGNANT TUMOR DISEASES

Introduction. The study of the possibility of using the circulating tumor cells (CTC) definition in the patients` blood with different localization of malignant tumors as a diagnostic criterion and the criterion of the effectiveness of specific treatment tactics is one of the topical issues in modern oncology.

The purpose of the paper is to analyze the results of using the developed information technology for identification of circulating tumor cells for the study of blood samples of patients in order to confirm or reject the initial diagnosis of cancer of different localization.

Results. Our information technology is based on the use of an advanced method of isolation of intact circulating cells, the difference of which is to supplement the structure of the basic ISET method (Isolation by Sizе of Tumor Cells) with new modes: 100% sealing chamber with hemolysate and providing it with the necessary and constant pressure during the filtration process by introducing a negative pressure gauge, as well as the mode of three-level filtering of the CTC on consecutive polycarbonate membranes with micropore diameters of 8 μm, 5 μm and 3 μm. To assess the malignancy of selected cells, the information technology used the method of determining the CTC according to the set of criteria, formed databases with created template CTC masks and control templates in the automated mode. Blood samples from patients were tested using IT. Taking into account each step of the technique (using different filters), analysis of the results showed that of the total proportion of samples, which additionally detected the CTC using not only an 8 μm filter, but also filters 5 μm and 3 μm, was 20.66 %.

Conclusions. The use of information technology to identify circulating tumor cells improves the efficiency of detecting these cells by reducing the testing time and expanding the range of research due to the ability to detect cells of small size. Improvement of IT by supplementing the knowledge base (complex of template mask masks and relevant expert findings) makes it possible to apply it in screening of patients’ blood, including at the preclinical stage of the examination.

Keywords: information technology, circulating tumor cells, method of isolation of circulating tumor cells, automated system, screening of patients’ blood.

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Recieved 11.12.2019