Chernyshova T.A. – CCE Journal

Issue 1 (195), article 6

DOI:https://doi.org/10.15407/kvt195.01.082

Kibern. vyčisl. teh., 2018, Issue 1 (195), pp.

Chernyshova T.A., physician,
e-mail: tetyana.che@gmail.com

Aviation Medical Center of the National Aviation University,
Komarova av., 1, Kyiv, 03058, Ukraine

CRITERIA AND METHOD FOR DETECTION OF CIRCULATING TUMOR CELLS

Introduction. Modern advances in science and technology have substantially expanded the possibilities for detecting malignant neoplasms. A great number of methods for the detection and allocation of circulating tumor cells clearly indicates the interest of researchers to this problem.
The purpose of the work is to form a complex of criteria for tumor malignancy evaluation and to improve the method of detecting circulating tumor cells in human blood.
Results. The proposed method for determining circulating tumor cells, which is an improvement of ISET technology, combines two stages. At the first stage the improvement is in adding two additional polycarbonate filters with 5 and 3 micron diameter pores, and providing a mode of 100% sealing of the chamber with hemolysis, and constant pressure throughout the filtration process. At the second stage, we carried out the determination of malignancy degree of the isolated cells using the developed set of criteria. The use of the developed method in the automated system for the analysis of digital microscopic images of circulating tumor cells provides the detection and calculation of characteristic features for assigning an object to a certain class of malignancy and the creation of scanned images database with recorded existing cells or their entities, as well as the final verification of the results of tumor malignancy evaluation for template masks of circulating tumour cells and benign tumor cells.
Conclusions. The application of the proposed method for the detection of circulating tumor cells allows detecting smaller cells than in case of using traditional methods, ensures their integrity and intactness.

Keywords: circulating tumor cells, criteria for evaluation of tumormalignancy, method of determining circulating tumor cells in human blood.

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Received 26.12.2018

Issue 2 (192), article 6

DOI:https://doi.org/10.15407/kvt192.02.084

Kibern. vyčisl. teh., 2018, Issue 2 (192), pp.

Zlepko S.M.¹,
D.Sc. (Engineering), Professor,
Head of the Department of Biomedical Engineering
e-mail: smzlepko@ukr.net
Chernyshova T.A.², Doctor
e-mail: tetyana.che@gmail.com
Maevsky O.E.³, Dr (Medical), Professor,
Head of the Department of Histology
e-mail: maevskyalex8@gmail.com
Krivonosov V.E.⁴, docent,
Department of Biomedical Engineering
e-mail: yhtverf007@ukr.net
Azarkhov O.Y., Dr (Medical), Professor,
Head of the Department of Biomedical Engineering
e-mail: azarhov55@mail.ru

¹Vinnytsia National Technical University,
Khm. highway 95, 21021, Vinnytsia, Ukraine
²Medical Center of National Aviation University,
Cosmonaut Komarov ave., 1, 03058, Kyiv, Ukraine
³Nicholay Pirogov Vinnitsa National Medical University,
Pyrohova str, 56, 21000, Vinnytsia, Ukraine
⁴Priazovsky State Technical University,
Universytetska str, 7, 87500, Mariupol, Ukraine

INFORMATION TECHNOLOGY OF DETERMINING CIRCULAR TUMOR CELLS IN HUMAN BLOOD

Introduction. The development of information systems and technologies for the processing of medical images of cells obtained in the study of histological preparations is one of the most important and priority directions of modern medical science.
The purpose of the article is to detect the CPR at various localizations of malignant neoplasms is currently one of the topical issues in oncology.
Results. A distinctive feature of the CPR is the aggressive metastatic potential, which allows them to be considered as the main mechanism of tumor progression. The article describes the methods of detecting the CPC, the functions and operations of image processing. The modern methods and algorithms for processing and restoring biomedical images are analyzed. The work of information technology for the determination of circulating tumor cells in human blood is given step by step. A comparison of the developed technology and existing analogues is made.
Conclusions. Unlike the existing technology, it detects a 4-micromycle GPC in the study of blood samples from patients with micellar lung cancer. The doctor, thus, received an automatic technology for the determination of the CPP in peripheral or venous blood with high reliability and informativeness, with maximum preservation of the integrity and invulnerability of circulating tumor cells. The analysis of literary sources and their own clinical studies have confirmed that only technologies based on the ISET method allow the detection of very rare circulating trophoblast cells of the fetus from the mother’s blood.

Keywords: technology, circulating tumor cell, medical image, histology, treatment, definition, criterion.

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Received 03.04.2018